VAMP-2 (vesicle associated membrane protein 2; also synaptobrevin-2) is a 13kD member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116aa in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa31-91), and a membrane-anchor domain (aa95-114). Over (aa1-94), human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.
Applications:
Suitable for use Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 0.1ug/ml
Immunohistochemistry: 25ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Myeloma cells with B cells from Balb/c mice.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay bufter.
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