A novel member of the vesicle-associated membrane protein (VAMP):synaptobrevin family, VAMP-3 is a v-SNARE protein that resides in recycling endosomes and endosome-derived transport vesicles. It colocalizes with endocytosed transferrin receptors and the glucose transporter GLUT4 in adipocytes, and is also present in alpha-granules in platelets. The protein has been implicated in recycling of transferrin receptors to the plasma membrane, secretion of alpha-granules in platelets, recycling of T-cell receptors to the immunological synapses, and membrane trafficking during cell migration. It is a homologue of VAMP-2 and has a broad tissue distribution in humans. Like VAMP-2, it can be cleaved by tetanus toxin, a metallo-endoprotease which blocks synaptic exocytosis. In humans, the VAMP-3 gene is mapped to chromosome 1p36.23.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
Human, mouse and rat brain lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.