Vanilloid Receptor Like Protein 1 (VRL-1) (Control Peptide)

Control peptide for V2075 (antiserum) and V2075-05 (affinity purified antibody). Synthetic peptide (18aa) corresponding to the C-terminus of the rat VRL-1 protein. No significant sequence homology is found with VR1 or other proteins. Species homogeneity: 100% conserved in mouse VRL-1 and growth factor regulated calcium channel. Only 52% homology with human VRL-1.\n\nNociception, the process of detecting noxious chemical, mechanical, or thermal stimuli, occurs predominantly at the peripheral terminal neurons known as polymodal nociceptors. Nociceptors transduce noxious stimuli into membrane depolarization that triggers action potential, conducts the action potential from the sensory sites to the synapses in the CNS, and conversion of action potentials invokes a perception of pain, discomfort, and appropriate mechanical/physical protective reflexes. At the molecular level, nociception is carried out by ion channels or receptors..Capsaicin receptor, termed vanilloid receptor subtype 1 (VR1), has been cloned from sensory neurons. VR1 protein is a heat-gated cation channel. VR1 is also activated by noxious heat (elevation in temperature causing pain) and high proton concentrations (pH <6.0). Rat VR1 encodes a membrane protein of 838 aa (~95kD) with 6 transmembrane domains. VR1 expression is limited to small to medium diameter primary sensory neurons. Recently, a structural homolog of VR1, termed VRL-1 (VR1 like proteins; rat 761 aa, human 764 aa) have been cloned and characterized. There is ~49% identity between VRL-1 and VR1. In contrast to VR1, VRL-1 does not respond to capsaicin, acid or moderate heat. Instead, it is activated by high temperatures with a threshold of ~52oC. Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium to large-diameter neurons, and in many non-neuronal tissues (lung, spleen, and intestine) that are unlikely to encounter temperatures of >50oC. It is possible that VRL-1 is also activated by stimuli other than heat.\n\nApplications:\nSuitable for use in ELISA or Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1ug/ml.\nAntibody Blocking: 5-10ug control peptide per 1ul V2075 (antiserum) or per 1ug V2075-05 (affinity purified antibody).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Freeze at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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