VAP-1 (Vascular Adhesion Protein 1, VAP1, Amine Oxidase Copper-containing 3 (Vascular Adhesion Protein 1), AOC3, Copper Amine Oxidase, HPAO, Membrane Copper Amine Oxidase, Semicarbazide-sensitive Amine Oxidase, SSAO, VP97)

VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. The majority of VAP-1 is stored within intracellular granules and translocated to the endothelial surface on inflammation. Structurally it belongs to enzymes called semicarbamizide-sensitive amine oxidases. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 possesses both adhesive and enzymatic properties in vitro. VAP-1 mediates leukocyte trafficking to sites of inflammation.\nThe monoclonal antibody 174-5 cross-reacts with rat VAP-1.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry on frozen sections. Useful for inhibition of biological activity. Inhibits lymphocyte infiltration in liver allograft rejection in rats.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2095-02D

Size

100ug

Applications

FC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Martelius, T et al; Blockade of vascular adhesion protein-1 inhibits lymphocyte infiltration in rat liver allograft rejection. Am J Path 2004, 165: 1993.

Additional Info

Recognizes human Vascular Adhesion Protein-1 (VAP-1) also known as AOC3, a heavily sialylated homodimeric glycoprotein of 180 kD.