VAPA (Vesicle-associated Membrane Protein-associated Protein A, VAMP-A, VAMP-associated Protein A, VAP-A, 33kD VAMP-associated Protein, VAP-33, VAPA, VAP33)

Supplier:

United States Biological

Cat no: V2024-02E

VAPA (Vesicle-associated Membrane Protein-associated Protein A, VAMP-A, VAMP-associated Protein A, VAP-A, 33kD VAMP-associated Protein, VAP-33, VAPA, VAP33)

VAP-A (VAMP-associated protein A; also VAP-33) is a 33kD integral membrane protein that is ubiquitously expressed and found associated with multiple cellular organelles, including the Golgi, endosomes, tight junctions, and the cell membrane. VAP-A appears to be pleiotrophic in function, participating in synaptic vesicle fusion, maintenance of tight junction integrity, and the regulation of cholesterol synthesis. Human VAP-A is a type IV (small luminal domain) transmembrane protein 249aa in length. It is almost all cytoplasmic (aa1-227) with a one aa luminal fragment. The cytoplasmic region contains an MSP/motile sperm protein domain (aa14-131) plus a coiled-coil region (aa169-205). VAP-A homo- and hetero-dimerizes with VAP-B. It has multiple binding partners within the cell. There is one potential alternative start site at Met8, and an isoform that shows a 44aa insertion after Leu140. Over aa1-132, human VAP-A shares 97% aa identity with mouse VAP-A.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.5-1ug/ml\nWestern Blot: 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2024-02E

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2A

Additional Info

Recognizes human VAP-A.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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