Vascular Endothelial Cadherin, phosphorylated (Tyr658) (VE-Cadherin, CD144)

VE-cadherin is an endothelial specific adhesion molecule that is essential for the maintenance of endothelial barrier function and angiogenesis. It is linked to the actin cytoskeleton through a number of adaptor proteins including alpha, beta, and gamma catenin. Cytoskeletal dynamics and phosphorylation regulate VE-cadherin mediated cell-cell adhesion. The interaction between cytosolic c-terminal Src kinase, SH2 domain and phosphorylated tyrosine 658 site of VE-Cadherin regulates density dependent cell proliferation. Phosphorylation of tyrosine 658 has been shown to lead to uncoupling of p120-catenin from the cytoplasmic tail of VE-cadherin. This phosphorylation event is sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis. \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000\nOptimal dilution to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2109-52C

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.3, 50% glycerol, containing 1 mg/ml BSA, and 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Baumeister, U., et al. (2005) Association of Csk to VE-cadherin and inhibition of cell \nproliferation. EMBO J. 24(9):1686-1695. \nvan Buul, J.D., et al. (2005) Proline-rich tyrosine kinase 2 (Pyk2) mediates vascular \nendothelial-cadherin-based cell-cell adhesion by regulating b-catenin tyrosine \nphosphorylation. J. Biol. Chem. 280(22):21129-21136. \nLambeng, N., et al. (2005) Vascular endothelial-cadherin tyrosine phosphorylation in \nangiogenic and quiescent adult tissues. Circ. Res. 96(3):384-391. \nHudry-Clergeon, H., et al.. (2005) Platelet-activating factor increases VE-cadherin tyrosine \nphosphorylation in mouse endothelial cells and its association with the PtdIns3i?-kinase. \nFASEB J. 19(6):512-520. \nEsser, S., et al. (1998) Vascular endothelial growth factor induces VE-cadherin tyrosine \nphosphorylation in endothelial cells. J. Cell Sci. 111:1853-1865.

Additional Info

The antibody detects a 120kD protein of VE-cadherin. Epitope: : Phosphotyrosine 658 \nSpecies Cross Reactivity: Human. Species Sequence Homology: This sequence is 100% homologous in mouse, rat, chicken, chimpanzee, cow, dog and pig.