Vascular Endothelial Growth Factor-165, Recombinant, Rat (VEGF-165, VEGF-A, VPF, Glioma-derived Endothelial Cell Mitogen)

Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilatation via nitric oxide-dependent pathways. VEGF-A has several variants, VEGF-165 being the most abundant. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95%. \n\nSource:\nRecombinant protein corresponding to two 165aa chains rat VEGF-165 expressed in E.coli. \n\nMolecular Weight: \n~38.8kD\n\nEndotoxin:\n(same/less than)1EU/ug (LAL)\n\nBiological Activity:\nThe activity is determined by the dose-dependent induced proliferation of Human umbilical vein endothelial cells (HUVECs) and is typically 4-8ng/ml.\n\nAA Sequence:\nMAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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