Vascular Endothelial Growth Factor, Recombinant, Mouse, aa27-214 (VEGF 188, VAS, Vasculotropin, VEGFA, VPF)

Vascular endothelial growth factor (VEGF or VEGFпїЅA), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1пїЅ3). It is a member of the PDGF family that is characterized by a cystine knot structure formed by eight conserved cysteine residues (4). Alternate splicing produces isoforms including 121, 145, 165, 183, 189, and 206 amino acid (aa) forms in humans, with 120, 164 and 188aa isoforms found in mouse (1пїЅ4). Mouse VEGF188 shares 98% aa sequence identity with the appropriate isoform in rat, and 89% with human, bovine and canine VEGF. While isoforms VEGF120 and VEGF121 are freely diffusible, VEGF188, VEGF189 and VEGF206 contain the highest number of basic aa, which bind heparin and tether these isoforms to the cell surface and extracellular matrix (3пїЅ5). Expression of VEGF188/189 is particularly high in the embryonic lung, where it is produced by type II alveolar epithelia (6). It is thought to be involved in lung, heart and liver vasculogenesis (5, 6). It is not sufficient for vasculogenesis during bone development, but may play a role in bone repair (5, 7). Tumor cell production of VEGF188/189 correlates with poor prognosis (5). VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called FltпїЅ1) and VEGF R2 (FlkпїЅ1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF188 binds VEGF R2 best when it is cleaved by uPA or plasmin into a 110пїЅ111aa form (4, 5). Human VEGF165 and VEGF189 also bind the semaphorin receptor NeuropilinпїЅ1 (8, 9).\n\nSource:\nRecombinant protein corresponding to aa27-214 from mouse VEGF 188, HEK293-derived.\n\nMolecular Weight:\n~28-30kD\n\nEndotoxin:\n<0.01EU/1ug (LAL method)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute in sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145722

Size

10ug

Form

Supplied as a lyophilized powder in HCl. BSA free. Reconstitute in 100ug/ml in 4mM HCl.

Purity

~95% (SDS-PAGE)

References

1. Breier, G. et al. (1992) Development 114:521. 2. Shima, D.T. et al. (1996) J. Biol. Chem. 271:3877. 3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777. 4. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853. 5. Woolard, J. et al. (2009) Microcirculation 16:572. 6. Ng, Y.S. et al. (2001) Dev. Dyn. 220:112. 7. Maes, C. et al. (2004) J. Clin. Endocrinol. 113:188. 8. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049. 9. Vintonenko, N. et al. (2011) Cell Adh. Migr. 5:332.