Vascular Endothelial Growth Factor, Recombinant, Mouse (VEGF)

VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant murine VEGF is a 39.0kD disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.\n\nBiological Activity: Determined by the dose-dependent \nstimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) \nusing a concentration range of 1.0-5.0ng/ml.\n\nAA Sequence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile dH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCountry of Origin: USA

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SPECIFICATIONS

Catalog Number

V2110-07

Size

2ug

Form

Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to a concentration of 0.1-1.0mg/ml which can\nfurther be diluted into other aqueous solutions.

Purity

(same/more than)98% by SDS-PAGE and HPLC analyses. Endotoxin: (same/less than) 0.1ng/ug (1EU/ug)