Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF)

Vascular endothelial growth factor (VEGF) was recently identified as a secreted, direct-acting mitogen specific for vascular endothelial cells and capable of stimulating angiogenesis in vivo. Molecular cloning revealed multiple forms of VEGF, apparently arising from alternative splicing of its RNA transcript. VEGF and TGF-beta1 are potent angiogenesis inducers with opposing effects on endothelial cells. TGF-beta1 induces apoptosis; VEGF protects endothelial cells from apoptosis. We found that TGF-beta1 promotes endothelial cell expression of FGF-2, which up-regulates VEGF synthesis. Inhibition of VEGF signaling through VEGF receptor 2 (flk-1) abrogates TGF-beta1-induced apoptosis and p38(MAPK) activation. This finding illustrates an unexpected role of VEGF and indicates that VEGF can be pharmacologically converted into an apoptotic factor, a novel approach to antiangiogenesis therapy.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-2ug/mL. Detects a band at ~38-44kD. \nELISA: 1:50-1:200\nImmunocytochemistry: 1:50-1:200\nImmunofluorescence: 1:50-1:200\nImmunohistochemistry (FFPE): Good results have been reported on human, bovine and pocine paraffin sections after microwave treatment and using Tris-HCl buffer, pH 10. Citrate buffer not recommended. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRecombinant human VEGF protein\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2110-08

Size

100ug

Applications

ELISA, ICC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Bov, Prc

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG1

References

1. Houck KA, et al. The vascular endothelial growth factor family: identification of a fourth molecular species and characterization of alternative splicing of RNA . Mol Endocrinol 5(12):1806-14, 1991.2. Ferrari G, et al. VEGF, a prosurvival factor, acts in concert with TGF-beta 1 to induce endothelial cell apoptosis. Proc Natl Acad Sci USA 103(46):17260-5, 2006.

Additional Info

Recognizes human VEGF. This antibody is highly specific, but only weakly stains transfected cells (5-10%). Species Crossreactivity: bovine and porcine (Immunohistochemistry only)