Vasopressin Receptor, Arg, V1a (AVP-V1a)

Vasopressin (AVP, Arginine-8-Vasopressin), the antidiuretic hormone, is a cyclic nonapeptide involved in the homeostasis of body fluid osmolality, blood volume, vascular tone, and blood pressure. Specific actions of AVP include inhibition of diuresis, contraction of smooth muscle, stimulation of liver glycogenesis, and modulation of ACTH release from pituitary. AVP belongs to the family of vasoactive and mitogenic peptide involved in normal and pathological cell growth and differentiation. AVP exerts its action through binding to specific membrane receptors coupled to distinct second messengers. There are 3 types of AVP receptors: V1a, V1b, and V2subtypes. The V2 receptor stimulates adenyl cyclase and protein Kinase A, V1 activate phospholipase A2.C, and D, resulting into production of IP3 and DAG, the mobilization of intracellular calcium, the influx of extracellular calcium, the activation of protein Kinase C, and protein phosphorylation. The V1a receptors mediate vasoconstriction and hepatic gluconeogenesis platelet aggregation, coagulation factor release. V1a receptors are found in vascular smooth muscle, hepatocytes, blood platelets, lymphocytes and monocyte, type II pneumocytes, adrenal cortex, brain, reproductive organs, retinal epithelium, renal mesangial cells.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested. \n\nRecommended Dilution: \nELISA: 0.5-1ug/ml. using 1ug/ml of V2120-30A control peptide /well.\nWestern Blot: 1-10ug/ml using Chemiluminescence technique.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2120-32C

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Morel, A. et al (1992) Nature 35: 523-526. 2. Innamorti, G. et al (1996) Biochem J. 314: 709-711. 3. Thibonnier, M. et al (1994) J Biol Chem. 269: 3304-10. 4. Hirasawa, A. et al (1994) BBRC 203: 72-79.

Additional Info

Recognizes rat AVP1a receptors ((394, 395, and 424 aa). Species sequence homology: mouse (84%), human (68%), sheep (52%). No significant homology with AVP V1b or V2 or V3 receptors.