Vasopressin Receptor V1a (AVR-V1a)

Rat AVR-V1a receptor is a G-protein coupled membrane protein with seven transmembrane domain. N-terminus is predicted to be extracellular. Vasopressin (AVP, Arginine-8-Vasopressin), the antidiuretic hormone is cyclic nonapeptide invovled in the homeostasis of body fluid osmolality, blood volume, vascular tone, and blood pressure. Specific actions of AVP include inhibition of diuresis, contraction of smooth muscle, stimulation of liver glycogenesis, and modulation of ACTH release from pituitary. AVP belongs to the family of vasocative and mitogenic peptide invovled in normal and pathological cell growth and differentiation. AVP receptors are members of the G-protein coupled receptors with putative 7 transmembrane domains. The sizes of various AVP receptors are V1a (rat, 424 AA; human, 418 AA); V1b (rat, 421 AA; human, 424 AA); V2 (rat, 371 AA; human 371 AA). The N-terminus and C-terminus are predicted to be extracellular and cytoplasmic, respectively.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:50,000\nWestern Blot: 1:1000-1:5000\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: \nV2120-30A Vasopressin Receptor V1a Control Peptide \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2120-30

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Neat serum

Isotype

IgG

References

1. Morel A et al (1992) Nature 356, 523-526. 2. Innamorti G et al (1996) Biochem J 314, 709-711. 3. Thibonnier, M et al (1994) J Biol Chem. 269, 3304-10. 4. Hirasawa A et al (1994) BBRC 203, 72-79.

Additional Info

Recognizes rat Arginine Vasopressin Receptor V1a (AVR-V1a). The immunogen shows no significant sequence homology with AVR-V1b, AVR-V2 or AVR-V3. Species sequence homology: mouse: 84%, human 68% and sheep 52%.