Vav, which is predominantly expressed during adult life, is one of the genetic markers that correlate with the differentiation of the hematopoietic cells. Its functional importance has been extensively studied in T and B cells, in which it appears crucial for both development and functions. In non-lymphoid hematopoietic cells, Vav is mainly involved in the response of mature cells to a variety of stimuli that lead to spatial and temporal reorganization of the actin-based cytoskeleton during proliferation and phagocytosis. Vav acts as a potential target for gene therapy of myeloproliferative disorders. Human Vav gene maps to the chromosome 19 in the region 19p13.2.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Positive Control:
Jurkat cells
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.