Vav1, phosphorylated (Tyr160) (GDP/GTP Exchange Factor)

Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases. The Vav family has currently three members in mammalian cells (Vav1, Vav2, and Vav3). Vav1 contains multiple protein binding motifs, indicating a role for Vav1as an adaptor protein linking cell surface receptors to downstream signaling proteins. Vav1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NFkB, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN-y, and TNF-a. Vav1 is also reported to activate JNK, p21-activated kinase (PAK) and PLCy, implicating its critical role in calcium mobilization, and cytoskeletal rearrangement. Activation of several cell surface receptors including integrins, immune response receptors, or growth factor receptors leads to phosphorylation of Vav1 on tyrosine residues including tyrosine 160.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562 cells expressing wild-type human Integrin av and b3 proteins\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2121-07A

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Houlard, M., et al. (2002) Vav1 is a component of transcriptionally active complexes. J. Exp. Med. 195(9):1115-1127. 2. Manetz, T.S., et al. (2001) Vav1 regulates phospholipase C gamma activation and calcium responses in mast cells. Mol. Cell Biol. 21(11):3763-3774. 3. Woodside, D.G., et al. (2001) Activation of Syk protein tyrosine kinase through interaction with integrin beta cytoplasmic domains. Curr. Biol. 11(22):1799-1804. 4. Bustelo, X.R. (2001) Vav proteins, adaptors and cell signaling. Oncogene 20(44):6372-6381. 5. Moores, S.L., et al. (2000) Vav family proteins couple to diverse cell surface receptors. Mol. Cell Biol. 20(17):6364-6373. 6. Costello, P.S., et al. (1999) The Rho-family GTP exchange factor Vav is a critical transducer of T- cell receptor signals to the calcium, ERK, and NF-kappaB pathways. Proc. Nat

Additional Info

Recognizes human Vav1 when phosphorylated at Tyr160. Species sequence homology: Other species (100%).