Vav2 (VAV2, vav 2 oncogene, Oncogene VAV2, Guanine nucleotide exchange factor, Gef)

Vav2 is an oncoprotein member of the Dbl family of proteins that serves as a guanine nucleotide exchange factor (GEF) for the Rho family of Ras-related GTPases (1). Vav2 possesses a C-terminal pleckstrin homology (PH) domain and a tandem Dbl homology (DH) domain which interacts with Rho family GTPases to catalyze GDP release (1-2). The PH\ndomain serves as a negative regulator of DH domain GEF activity, which is promoted by phosphatidylinositol 4, 5-phosphate (PIP2) and is antagonized by the PI3K product, phosphatidylinositol3, 4, 5-phosphate (PIP3) (3). Hence, PI3K may facilitate the activation of Vav. Vav2 also contains a cysteine-rich domain (CRD) that is a positive modulator for DH\ndomain function (4). Vav2 is widely expressed in human tissues (5-6). The SH2 domain of Vav2 associates with the receptor tyrosine kinases EGFr (7-9) and PDGFr (8), binding preferentially to the phosphorylated residues pTyr-992 and pTyr-1148 of EGFr (10) and directly to multiple autophosphorylation sites of PDGFr (8). Overexpression of Vav2 has been shown to be sufficient to activate its oncogenic potential (6). In ovarian tumor cells, the binding of hyaluronan to its receptor (CD44v3) and the recruitment of Grb2 and p185HER2 to the CD44v3-Vav2 complex activates Vav2-mediated Rac1 and Ras signaling, leading to ovarian tumor cell migration and growth.\n\n\nDomain Information: Calponin homology domain; PH domain; Phorbol esters/diacylglycerol binding domain (C1 domain); RhoGEF domain; Src homology 2 domains; Src homology 3 domains\n\nGene Ontology Terms: guanyl-nucleotide exchange factor activity; intracellular signaling cascade; diacylglycerol binding\n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 100kD band observed in 293 lysate (predicted MW of 100kD according to NP_003362). Recommended for use at 1-1.5ug/ml.\n\nStaining (1.5ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See V2121-11A-P. Antibody

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SPECIFICATIONS

Catalog Number

V2121-11A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Henske EP et al. Identification of VAV2 on 9q34 and its exclusion as the tuberous sclerosis gene TSC1. Ann Hum Genet 59 ( Pt 1) :25-37 (1995). PubMed: 7762982.\nGeneral References:\n1. Bustelo, X.R., Mol. Cell Biol. 20: 1461-1477, 2000. 2. Cerione, R.A., Zheng, Y., Curr. Opin. Cell Biol. 8: 216-222, 1996. 3. Han, J., et al., Mol. Cell Biol. 17: 1346-1353, 1997. 4. Booden, M., et al., J. Mol. Cell. Biol. 22: 2487-2497, 2002. 5. Henske, E.P., et al., Ann. Hum. Genet. 59: 25-37, 1995. 6. Schuebel, K.E., et al., Oncogene 13: 363-371, 1996. 7. Liu, B.P., Burridge, K., Mol. Cell. Biol. 20: 7160-7169, 2000. 8. Moores, S.L., et al., Mol. Cell Biol. 20: 6364-6373, 2000. 9. Pandey, A., et al., PNAS USA 97: 179-184, 2000. 10. Tamas, P., et al., J. Biol. Chem. 25: 25, 2002. 11. Bourguignan, L.Y.W., et al., J. Biol. Chem. 276: 48679-48692, 2001.

Additional Info

Recognizes Human, Mouse VAV2