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Vav3 (vav 3 oncogene, Oncogene VAV23 Guanine nucleotide exchange factor, Gef, GDP/GTP Exchange Factor, Gef)

Cat no: V2121-15B

Vav3 (vav 3 oncogene, Oncogene VAV23 Guanine nucleotide exchange factor, Gef, GDP/GTP Exchange Factor, Gef)

Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases. The VAV family has currently three members in mammalian cells (VAV 1, VAV 2, and VAV 3). VAV 1 contains multiple protein binding motifs, indicating a role for VAV 1 as an adaptor protein linking cell surface receptors to downstream signaling proteins. VAV 1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NF(kappa)B, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN lambda, and TNF alpha. VAV 3 is also involved in activating JNK, p21-activated kinase (PAK) and PLC lambda, implicating a critical role in cytoskeletal rearrangement. VAV 3 is inducibly tyrosine phosphorylated in response to stimulation of the epidermal growth factor (EGFR), platelet-derived growth factor (PDGFR) and T-cell receptors (TCR), enhancing its guanine nucleotide exchange factor activity in vitro. Tyrosine 173 is the regulatory phosphorylation site of VAV 3. \n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunoblot Analysis: 0.5-2ug/ml of this lot detected mouse Vav3 in Jurkat cells transfected with murine Vav3.\nImmunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate Vav3 from primary mouse thymocytes and overexpressed myc-tagged murine Vav3 in Jurkat cell lysate.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2121-15B

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Cao, Y., et.al., EMBO J. 21: 4809-4819, 2002. 2. Turner, M. and D.D. Billadeau, Nature Rev. 2: 476-486, 2002.

Additional Info

Recognizes Vav3, Mr ~98kD. Species Crossreactivity: Mouse. Slight crossreactivity to human.

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