Vav3 (vav 3 oncogene, Oncogene VAV23 Guanine nucleotide exchange factor, Gef, GDP/GTP Exchange Factor, Gef)

Vav proteins belong to the guanidine nucleotide exchange factor family of proteins. These proteins couple receptors to Rho-GTPases. To date, three members of the Vav family have been identified in mammalian cells: Vav1, Vav2 and Vav3. Vav proteins contain multiple protein binding motifs and act as adaptor proteins linking cell surface receptors to downstream signaling proteins. Vav3 transcripts are ubiquitously expressed in wide variety of cell lines including hematopoietic cells, suggesting its role in lymphocyte development and antigen receptor mediated activation of NFAT and NFkB. Vav3 is also involved in activating JNK, p21-activated kinase (PAK) and PLCy, implicating a critical role in cytoskeletal rearrangement. Vav3 is inducibly tyrosine phosphorylated in response to stimulation of the epidermal growth factor (EGFR), platelet-derived growth factor (PDGFR) and T-cell receptors (TCR), enhancing its guanine nucleotide exchange factor activity in vitro. Tyrosine 173 is the regulatory phosphorylation site of Vav3.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nCos7 cells overexpressing Vav3, +/- epidermal growth factor (EGF.)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2121-16D

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Sachdev, P., et al. (2002) Distinct role of phosphatidylinositol 3-kinase and Rho family GTPases in Vav3-induced cell transformation, cell motility, and morphological changes. J. Biol. Chem. 77(20):17638-17648. 2. Fujikawa, K., et al. (2002) Vav3 is regulated during the cell cycle and effects cell division. Proc. Nat

Additional Info

Recognizes mouse Vav3. Species sequence homology: Human (100%) and chicken (92%).