VCAM-1 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse VCAM-1 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nVascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9kD. As a result of glycosylation, the molecular mass of 120kD is revealed by SDS-PAGE.\n\nAssay Range:\n156pg/ml-10,000pg/ml\n\nSensitivity:\n< 5 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP29533\n\nEntrez Gene ID:\n22329\n\nKit Components:\n96-well plate pre-coated with anti-VCAM-1, Mouse\nLyophilized recombinant VCAM-1, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144093

Size

96Tests

References

1. Cybulsky, M. I.; Fries, J. W. U.; Williams, A. J.; Sultan, P.; Eddy, R.; Byers, M.; Shows, T.; Gimbrone, M. A., Jr.; Collins, T. Gene structure, chromosomal location, and basis for alternative mRNA splicing of the human VCAM1 gene. Proc. Nat. Acad. Sci. 88: 7859-7863, 1991. \n2. Besemer, J.; Harant, H.; Wang, S.; Oberhauser, B.; Marquardt, K.; Foster, C. A.; Schreiner, E. P.; de Vries, J. E.; Dascher-Nadel, C.; Lindley, I. J. D. Selective inhibition of cotranslational translocation of vascular cell adhesion molecule 1. (Letter) Nature 436: 290-293, 2005. \n3. Garmy-Susini, B.; Jin, H.; Zhu, Y.; Sung, R.-J.; Hwang, R.; Varner, J. Integrin alpha-4-beta-1--VCAM-1--mediated adhesion between endothelial and mural cells is required for blood vessel maturation. J. Clin. Invest. 115: 1542-1551, 2005.\n