VCAM-1, Recombinant, Human (CD106 Antigen, DKFZp779G2333, INCAM 100, INCAM-100, L1CAM, MGC99561, Vascular Cell Adhesion Molecule 1, VCAM 1, VCAM1, CD106, Vascular Cell Adhesion Protein 1)

VCAM is a 110kD cell surface integral membrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. The primary function of VCAM-1 is the mediation of leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 may play a vital role in the development several diseases, including atherosclerosis and rheumatoid arthritis. The human VCAM-1 gene codes for a 715aa transmembrane glycoprotein containing a 19aa cytoplasmic domain, a 22aa transmembrane domain, and a 674aa extracellular domain. Recombinant human VCAM-1 is a 74kD glycoprotein comprising the extracellular domain (674aa residues) of VCAM-1. Monomeric glycosylated VCAM-1 migrates at an apparent molecular weight of approximately 90kD by SDS-PAGE analysis under reducing conditions.\n\nBiological Activity:\nDetermined by its ability to support the adhesion of human U937 cells. The expected ED50 for this effect is 0.8-1ug/ml.\n\nAA Sequence:\nFKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYSF PKDPEIHLSG PLEAGKPITV KCSVADVYPF DRLEIDLLKG DHLMKSQEFL EDADRKSLET KSLEVTFTPV IEDIGKVLVC RAKLHIDEMD SVPTVRQAVK ELQVYISPKN TVISVNPSTK LQEGGSVTMT CSSEGLPAPE IFWSKKLDNG NLQHLSGNAT LTLIAMRMED SGIYVCEGVN LIGKNRKEVE LIVQEKPFTV EISPGPRIAA QIGDSVMLTC SVMGCESPSF SWRTQIDSPL SGKVRSEGTN STLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFPR DPEIEMSGGLV NGSSVTVSCK VPSVYPLDRLE IELLKGETILE NIEFLEDTDM KSLENKSLEMT FIPTIEDTGKA LVCQAKLHID DMEFEPKQRQ STQTLYVNVA PRDTTVLVSP SSILEEGSSV NMTCLSQGFP APKILWSRQL PNGELQPLSE NATLTLISTK MEDSGVYLCE GINQAGRSRK EVELIIQVTP KDIKLTAFPS ESVKEGDTVI ISCTCGNVPE TWIILKKKAE TGDTVLKSID GAYTIRKAQL KDAGVYECES KNKVGSQLRS LTLDVQGREN NKDYFSP\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

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