VDAC1 (Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM)

The voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane is a small, \nabundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic \nmitochondria. The VDAC protein is thought to form the major pathway for movement of adenine \nnucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and \nglycerol kinase. At low transmembrane voltage, VDAC is open for anions such as phosphate, chloride, \nand adenine nucleotides. At higher transmembrane voltage, VDAC functions as a selective channel for \ncations and uncharged molecules. These features make VDAC likely to play a role in mitochondrial \nenergy metabolism. Huizing et al. studied by Northern and Western blot analyses the human tissue \ndistribution of mitochondrial transmembrane metabolite carriers. They found that VDAC1 mRNA has a \nubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas \nthe VDAC2 isoform appears to be expressed only in the heart.\n\nUniProt Number:\nP21796\n\nGene ID:\nVDAC1\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen), and Immunocytochemistry.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145349

Size

100ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Blachly-Dyson, E., Zambronicz, E. B., Yu, W. H., Adams, V., McCabe, E. R. B., Adelman, J., \nColombini, M., Forte, M.Cloning and functional expression in yeast of two human isoforms of the \nouter mitochondrial membrane channel, the voltage-dependent anion channel.J. Biol. Chem. 268: \n1835-1841, 1993.\n2. Geisler, S., Holmstrom, K. M., Skujat, D., Fiesel, F. C., Rothfuss, O. C., Kahle, P. J., Springer, \nW.PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1.Nature Cell Biol. \n12: 119-131, 2010.\n3. Huizing, M., Ruitenbeek, W., van den Heuvel, L. P., Dolce, V., Iacobazzi, V., Smeitink, J. A. M., \nPalmieri, F., Trijbels, J. M. F.Human mitochondrial transmembrane metabolite carriers: tissue \ndistribution and its implication for mitochondrial disorders.J. Bioenerg. Biomembr. 30: 277-284, \n1998.

Additional Info

Recognizes human, mouse and rat VDAC1. No crossreactivity with other proteins.