p115 / TAP (Vesicle docking protein p115, Transcytosis-associated protein) is a general vesicular transport factor located in the Golgi complex. p115 is part of the Golgin protein family, defined by their location in the Golgi matrix and their long coiled-coil domain. p115 is thought to be essential for ER to Golgi traffic (1). Specifically, p115 is required for intercisternal transportation within the Golgi stack and required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane (2). Also, p115 has been suggested to be act as a vesicular anchor to the target membrane.
Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500
Immunoprecipitation: 1:30
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.