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VDR (NR1I1, Vitamin D3 receptor, 1,25-dihydroxyvitamin D3 receptor)

Cat no: 136518

VDR (NR1I1, Vitamin D3 receptor, 1,25-dihydroxyvitamin D3 receptor)

The vitamin D receptor, a NR1 Thyroid Hormone-Like Receptor, is activated by calcitriol, the active hormonal form of vitamin D. The activated receptor binds DNA at a specific vitamin D-responsive element and promotes transcription of vitamin D target genes such as osteocalcin, the most abundant noncollagenous protein in bone. The vitamin D receptor affects growth, bone formation, and female reproduction. For example, this receptor modulates the growth and differentiation of keratinocytes and mediates the beneficial effects of vitamin D on psoriatic skin. It also inhibits hormone secretion by and proliferation of parathyroid cells. The vitamin D receptor functions either as a homodimer or as a heterodimer of vitamin D and retinoid acid X receptor subunits. Inactivation of the receptor leads to hypocalcemia and Rickets-alopecia syndrome. Recently, the vitamin D receptor has been shown to function as a receptor for the secondary bile acid lithocholic acid (LCA), which is hepatotoxic and a potential enteric carcinogen. Activation of VDR by LCA or vitamin D induced expression in vivo of CYP3A, a cytochrome P450 enzyme that detoxifies LCA in the liver and intestine. An alternatively spliced vitamin D receptor that has an altered ligand-binding domain has been isolated from rats.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:100-1:500 \nELISA: 1:10,000 \nWestern Blot: 1:500-1:1000 \nImmunohistochemistry (Paraffin): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136518

Size

50ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human VDR.

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