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VDR (Vitamin D3 Receptor, 1,25-dihydroxyvitamin D3 Receptor, Nuclear Receptor Subfamily 1 Group I Member 1, NR1I1)

Cat no: V2130-01E

VDR (Vitamin D3 Receptor, 1,25-dihydroxyvitamin D3 Receptor, Nuclear Receptor Subfamily 1 Group I Member 1, NR1I1)

The vitamin D receptor, a NR1 Thyroid Hormone-Like Receptor, is activated by calcitriol, the active hormonal form of vitamin D. The activated receptor binds DNA at a specific vitamin D-responsive element and promotes transcription of vitamin D target genes such as osteocalcin, the most abundant noncollagenous protein in bone. The vitamin D receptor affects growth, bone formation, and female reproduction. For example, this receptor modulates the growth and differentiation of keratinocytes and mediates the beneficial effects of vitamin D on psoriatic skin. It also inhibits hormone secretion by and proliferation of parathyroid cells. The vitamin D receptor functions either as a homodimer or as a heterodimer of vitamin D and retinoid acid X receptor subunits. Inactivation of the receptor leads to hypocalcemia and Rickets-alopecia syndrome. Recently, the vitamin D receptor has been shown to function as a receptor for the secondary bile acid lithocholic acid (LCA), which is hepatotoxic and a potential enteric carcinogen. Activation of VDR by LCA or vitamin D induced expression in vivo of CYP3A, a cytochrome P450 enzyme that detoxifies LCA in the liver and intestine. An alternatively spliced vitamin D receptor that has an altered ligand-binding domain has been isolated from rats.\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2130-01E

Size

50ug

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

Additional Info

Recognizes human VDR.

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