VEGF-C BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human VEGF-C in cell culture supernates, serum and plasma (heparin, EDTA).\n\nVascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D (VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein (BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23kD after glycosylation.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 3 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP49767\n\nEntrez Gene ID:\n7424\n\nKit Components:\n96-well plate pre-coated with anti-VEGF-C, Human\nLyophilized recombinant VEGF-C, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144098

Size

96Tests

References

1. Joukov, V.; Pajusola, K.; Kaipainen, A.; Chilov, D.; Lantinen, I.; Kukk, E.; Saksela, O.; Kalkkinen, N.; Alitalo, K. : A novel vascular endothelial growth factor, VEGF-C, is a ligand for the Flt4 (VEGFR-3) and KDR (VEGFR-2) receptor tyrosine kinases. EMBO J. 15: 290-298, 1996.\n2. Lee, J.; Gray, A.; Yuan, J.; Luoh, S.-M.; Avraham, H.; Wood, W. I. : Vascular endothelial growth factor-related protein: a ligand and specific activator of the tyrosine kinase receptor Flt4. Proc. Nat. Acad. Sci. 93: 1988-1992, 1996.\n