VEGF R2 (FLK1/KDR)

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1:50-1:100\nWestern Blot: 1:500-1:2000\nImmunohistochemistry(Frozen/Paraffin): 1:50-1:100 (ABC)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2110-02F

Size

100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Ankoma-Sey V et al. Coordinated induction of VEGF receptors in mesenchymal cell types during rat hepatic wound healing. Oncogene 17:115-21 (1998). 2. Ergun S et al. Vascular endothelial growth factor and its receptors in normal human testicular tissue. Mol Cell Endocrinol 131:9-20 (1997). 3. Quinn TP et al. Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium. Proc Natl Acad Sci U S A 90:7533-7 (1993).

Additional Info

Recognizes the native form of mouse VEGF R2, 180-190kD. Species Crossreactivity: human and rat