VEGF Receptor 1 (Flt1, VEGFR1) Soluble (Vascular Endothelial Growth Factor Receptor 1, sVEGFR-1, sFlt1)

VEGFR-1 (vascular endothelial growth factor receptor 1) is a 180-kDa membrane receptor tyrosine kinase that is the receptor for vascular endothelial growth factor (VEGF), VEGFB and PGF. VEGF is a key regulator of physiological angiogenesis during embryogenesis, skeletal growth and reproductive functions. VEGF has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. VEGFR-1 has seven extracellular Ig-like domains; the second domain from the N-terminus is necessary and sufficient for high affinity VEGF binding. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2, and two major tyrosine phosphorylation sites at Tyr 1213 and Tyr 1242 and two minor tyrosine phosphorylation sites at Tyr 1327 and Tyr 1333 exist in VEGFR-1.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, ELISA and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (Paraffin): 1: 250\nImmunocytochemistry: 1: 250\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2110-16E

Size

100ul

Applications

ELISA, ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Ferrara, N. et al. The biology of VEGF and its receptors. Nat. Med. 9: 669

Additional Info

Recognizes human VEGFR1 at ~180kD. Detects both VEGFR1 and its splice isoform sFlt1. Species Crossreactivity: mouse and rat (tested in Western Blot only)