VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)

The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms- like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co- operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions: \nImmunohistochemistry (Paraffin): 1:50-1:500. Use standard deparaffinization techniques on tissue speciens. Preheat tissues using a citrate buffer, pH 6.0 and high heat epitope retrieval techniques. Note: Do not allow tissues to dry out during the staining procedure.\nOptimal dilutions to be determined by the researcher.\n\nControl:\nPancrea, Kidney, Adenocarcinoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2110-05

Size

100ug

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Rabbit

Form

Supplied as a liquid in 10mM PBS, pH 7.4.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Mukhopadhyay, D., et al., Nature 375: 577

Additional Info

Recognizes 34-54kD isoforms of human Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF). \nSpecies Crossreactivity: rabbit, mouse and rat