VEGFR2/KDR BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human VEGFR2/KDR in cell culture supernates, serum and plasma (heparin, EDTA).\n\nVascular endothelial growth factor receptor 2 (VEGFR-2) also known as Kinase insert domain receptor (KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 4 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP35968\n\nEntrez Gene ID:\n3791\n\nKit Components:\n96-well plate pre-coated with anti-VEGFR2/KDR, Human\nLyophilized recombinant VEGFR2/KDR, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144099

Size

96Tests

References

1. Shay-Salit, A., Shushy, M., Wolfovitz, E., Yahav, H., Breviario, F., Dejana, E., Resnick, N. VEGF receptor 2 and the adherens junction as a mechanical transducer in vascular endothelial cells. Proc. Nat. Acad. Sci. 99: 9462-9467, 2002.\n2. Sait, S. N. J., Dougher-Vermazen, M., Shows, T. B., Terman, B. I. The kinase insert domain receptor gene (KDR) has been relocated to chromosome 4q11-q12. Cytogenet. Cell Genet. 70: 145-146, 1995.\n