Velis 3 (MALS 3)

Protein assembly at the post synaptic density (PSD) of neuronal synapses is mediated in part by protein interactions with PDZ (PSD-95/discs large/zona occludens-1) motifs.The Mamamalian LIN-7 (MALS) proteins(1) are a family of small synaptic proteins that are the mammalian homologs of LIN-7, a C. elegans protein essential for vulval development. MALS proteins are also known as Veli (Vertebrate Lin-7) proteins.(2) MALS-3 mRNA is most abundant in kidney, followed by brain, liver, thymus and heart. MALS-3 is undetectable in spleen. In cultured cortical neurons, MALS proteins are clustered together with PSD-95 and NMDA-type glutamate receptors. MALS proteins are suggested to have roles in regulating the recruitment of neurotransmitter receptors to the PSD(1) and in the assembly of proteins involved in synaptic vesicle exocytosis and synaptic junctions.(2) \n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1.0ug/ml \nWestern Blot: 1ug/ml \nImmunohistochemistry (frozen): 1ug/ml \nImmunoprecipitation: 1ug/IP reaction\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nVelis-3 transfected COS cells and rat brain lysates \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2121-45

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Jo K, Derin R, Li M, Bredt DS. Characterization of MALS/Velis-1,-2, and-3: a family of mammalian LIN-7 homologs enriched at brain synapses in association with the postsynaptic density-95/NMDA receptor postsynaptic complex. J. Neurosci., June 1; 19(11):4189

Additional Info

Recognizes rat Velis 3 at 22kD. Crossreactivity with recombinant Velis-1 or Velis-2 proteins or with other related endogenous proteins has not been observed.