Verotoxin, BioAssay(TM) ELISA Kit

The Verotoxin (VT), or shiga-like toxin family is a group of closely related toxins produced by certain strains of Escherichia coli. These strains are a significant cause of human hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). In addition, they are both water borne and food borne and may also be transmitted from person-to-person by the oral-fecal route. In adults, illness caused by Verotoxin may last several days, while in children and the elderly, the illness can be fatal.\n\nInfections occur as sporadic illnesses or outbreaks, and are distributed throughout North and South America, Europe, and Asia. The peak incidence of infection occurs during summer months. In the past, lengthy and cumbersome diagnostic procedures have been utilized for the detection of verotoxin. Currently, simple to perform enzyme-linked immunosorbent assays (ELISA) are being used as an alternative to these longer tedious methods. These antigen-detection assays provide a cost effective tool for the management of diarrheal disease with an accurate and rapid test.\n\nKit Components:\n1. Microtiter plate 1x96 wells each\n2. Test strip holder: 1 (one)\n3. Negative Control: 1 vial x 2ml of a buffered base.\n4. Positive Control: 1 vial x 2ml of inactivated Verotoxin antigen in a buffered base.\n5. Reagent 1: 1x11ml of monoclonal anti-Verotoxin antibodies in a buffer with preservative.\n6. Reagent 2: 1x11ml of an anti-mouse antibody conjugated to horseradish\nperoxidase in a buffer with preservative.\n7. Chromogen: 1x11ml of the chromogen tetramethylbenzidine (TMB).\n8. Wash concentrate solution (20x): 2x25ml of concentrated buffer and surfactant with preservative.\n9. Stop solution: 1x11ml of 1M phosphoric acid.\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2121-69

Size

1Kit

Applications

ELISA

References

1. Johnson, R.P., et al., Infection and Immunity. 64(5): 1879-1883 (1996). 2. Gray, L.D., \"Escherichia, Salmonella, Shigella, and Yersinia\". Manual of Clinical Microbiology. 6th edition. American Society for Microbiology, pp.450-453. 3. Martin, I.E., et al., Journal of Clinical Microbiology. 34(3): 720-723 (1996). 4. Ramotar, K., et al., Journal of Clinical Microbiology 33(5): 1114-11200 (1995). 5. Karch, H., et al., Journal of Clinical Microbiology 34(3): 516-519 (1996). 6. Park, C.H., et al., Journal of Clinical Microbiology 34(4): 988-990 (1996). 7. Law, D. & Kelly, J., Infection and Immunity 63(2): 700-702 (1995). 8. Ramotar, K., et al., Journal of Clinical Microbiology 33(3): 519-524 (1995). 9. Park, C.H. & Hixon, D.L., \"Evaluation of a Commercial ELISA Test for Detection of Escherichia coli 0157 Antigen directly from Stool Specimens\". Recent Advances in Verocytotoxin-producing Escherichia coli Infections. International Congress Series 1072. pp.97-100. 10. Dylla, B.L., et al., Journal of Clinical Microbiology 33(1): 222-224 (1995). 11. Caprioli, A., et al., The Journal of Infectious Diseases 169(1): 208-211 (1994). 12. Chart, H., Serodiagn. Immunother. Infect. Disease. 1: 8-12 (1993).