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Verotoxin I-B subunit (Shiga-like, SLT-1b)

Verotoxin I-B subunit (Shiga-like, SLT-1b)

Cat no: V2121-60B

Supplier: United States Biological
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Verotoxins are also called Shiga-like toxins (SLTs). Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share > 99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis. Applications: Suitable for use in Western Blot, ELISA and Inhibition. Other applications not tested. Recommended Dilution: Western Blot: 1:10 Inhibition: in vitro dilutions should be made according to the amount of toxin to be inactiviated. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: V2121-60B
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1% BSA.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography
Alternative names: Verotoxin 1
References: 1. Strockbine, N et al; Characterization of monoclonal antibodies against Shiga-like toxin from Escherichia coli. Infect Immun 1985, 50: 695. 2. Smith, M et al; Development of a hybrid Shiga holotoxoid vaccine to elict heterologous protection against Shiga toxins types 1 and 2. Vaccine 2006, 24:4122. 3. Smith, M et al; The 13C3 monoclonal antibody that neutralizes Shiga toxin type 1 (Stx1) recognizes three regions on the Stx1 subunit and prevents Stx1 from binding to its eukaryotic receptor globotriaosylceramide. Infect Immun 2006, 74:6992.
Additional info: Recognizes the 1-B subunit of Verotoxin (Shiga-like toxin-1).

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