Verotoxin II A subunit (Shiga-like toxin 2 subunit A, SLT-2a, SLT-IIa)

Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11, and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share >99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. Antibodies to SLT-II can also neutralize a variant of SLT-II (designated SLT-Iiv) produced by strains of E.coli that cause edema. SLT-IIv is cytotoxic for Vero but not HeLa cells, distinguishing it from SLT-II. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis. \n \nApplications:\nSuitable for use in Western Blot, immuno assays,and for inhibition of the biological activity of the toxin. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2121-60C

Size

100ug

Applications

WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA

P Type

Mab

Purity

Purified by Protein G affinity chromatography

Isotype

IgG1

References

1. Perera, L et al; Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J Clin Microbiol 1988, 26: 2127 2. Smith, M et al; Development of a hybrid Shiga holotoxoid vaccine to elict heterologous protection against Shiga toxins types 1 and 2. Vaccine 2006, 24:4122 3. Smith, M et al; The 13C3 monoclonal antibody that neutralizes Shiga toxin type 1 (Stx1) recognizes three regions on the Stx1 subunit and prevents Stx1 from binding to its eukaryotic receptor globotriaosylceramide. Infect Immun 2006, 74:6992

Additional Info

Recognizes the 2A subunit of Shiga-like toxin 2

Alternative Names

Verotoxin 2