Home  >  Products  >  Vesicle Protein Sorting 35 (Vacuolar Protein Sorting-associated Protein 35, VPS35, hVPS35, Maternal-embryonic 3, MEM3, TCCCTA00141)

Vesicle Protein Sorting 35 (Vacuolar Protein Sorting-associated Protein 35, VPS35, hVPS35, Maternal-embryonic 3, MEM3, TCCCTA00141)

Cat no: V0650-24A


Supplier: United States Biological
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VPS35, otherwise known as MEM3, is a ubiquitously expressed member of the vacuolar protein sorting (VPS) family, and vital component of the multimeric retromer complex, involved in the retrieval of lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans Golgi network. Applications: Suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Brain lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: V0650-24A
Reactivities: Human
Hosts: Goat
Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: Vacuolar Protein Sorting 35 Homolog (S. cerevisiae), DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388
References: 1. Zhang P, Yu L, Gao J, Fu Q, Dai F, Zhao Y, Zheng L, Zhao S. Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21. Genomics. 2000 Dec 1;70(2):253-7. PMID: 11112353
Additional info: Recognizes human VPS35/MEM3. Species sequence homology: mouse and rat.

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