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Vesicular Monoamine Transporter 1, CT (VMAT1)

Cat no: V2121-88

Vesicular Monoamine Transporter 1, CT (VMAT1)

VMAT1 has been localized in adrenal chromaffin cells, endocrine and paracrine cells associated with the intestine, stomach and sympathetic nervous system (2,3). VMAT1 has also been found in endosomes of transfected CHO cells and in LDCVs of rat PC12 cells (2).\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml (Chemiluminescence technique). Detects a major band at ~55kD in postnuclear supernatants of CHO transfected with VMAT1 and not in wild type cells. Some additional bands, both higher and lower MW, were also detected and remain unaffected by the inclusion of protease inhibitors. \nImmunohistochemistry: 1-20ug/ml on paraformaldehyde fixed sections. \nELISA: 0.5-1.0ug/ml (1ug/ml VMAT1 peptide/well) \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2121-88

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1) Cell (1992) 70:539

Additional Info

Recognizes rat Vesicular Monoamine Transporter 1, CT. Species Crossreactivity: mouse. Not expected to crossreact with human VMAT1.

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