Vesicular Monoamine Transporter 1 (VMAT1)

The regulated exocytotic release of neurotransmitters in response to neural activity requires storage within intracellular vesicles. In the nervous system, these vesicles are the synaptic vesicles that are derived from the endosomal compartment, whereas in endocrine cells larger secretory granules, such as the chromaffin granules of adrenal medulla, are derived from the trans golgi networks. For classical transmitters that are synthesized in the cytoplasm or appear there after removal form the synapses by plasma membrane reuptake, storage depends upon the active transport into the vesicles. Several distinct transport activities have been identified for monoamines, acetylcholine, glutamate, GABA and glycine. Vesicular monoamine transporters (VMATs) catalyze transport and storage of monoamines, serotonin, dopamine, norepinephrine, epinephrine, and histamine. The driving force utilized by the VMAT is the H+ electrochemical gradient generated by a vacuolar ATP-dependent H+ pump (V-ATPase) located on vesicular plasma membrane. VMAT is inhibited by a wide variety of compounds including reserpine and tetrabenazine. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000 (ECL)\nELISA: 1:10,000-50,000. Control peptide (Cat #V2121-88B) can be used to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2121-88D

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Liu, Y et al (1992) Cell 70, 539-551. 2. Liu Y et al (1994) J Cell Biol. 127, 1419-1433 3. Weihe E et al (1994) J. Mol. Neurosci. 5, 149-164 4. Erickson JD et al (1996) Proc. Natl. Acad. Sci. 93, 5166-5171

Additional Info

Recognizes rat VMAT1. Does not recognize VMAT2 in transfected cells . The C-terminal rat VMAT1 sequence does not show any appreciable homology with hVMAT1.