VG5Q (Angiogenic Factor) (FLJ10283, HSU84971, HUS84971, Vasculogenesis gene on 5q)

VG5Q functions as an angiogenic factor in promoting angiogenesis and suppression of VG5Q expression inhibits vessel formation. Angiogenic factors are critical to the initiation of angiogenesis and maintenance of the vascular network. Angiogenesis has an essential role in pathological conditions such as cancer and various ischaemic and inflammatory diseases. VG5Q can bind to endothelial cells and promote cell proliferation, suggesting that the protein may act in an autocrine fashion. VG5Q interacts with TWEAK (also known as TNFSF12), another secreted angiogenic factor. VG5Q shows strong expression in blood vessels and is secreted when vessel formation is initiated. VG5Q protein was detected mostly in the cytoplasm and around the nuclei of human microvascular endothelial cells (HMVECs). Furthermore VG5Q is detected in human umbilical vein endothelial cells (HUVECs), human heart fibroblast (HHF) and ovarian cancer cells (OV-3), but low expression was detected in kidney cancer cells (RP-45), HeLa Cells and bladder cancer cells.\n\nApplications:\nUseful for Immunoprecipitation and Immunohistology on both frozen and paraffin sections. Furthermore the polyclonal antibody can be used for immunoassays and Western Blot.\n\nRecommended Dilution:\nWestern Blot: 1:10\nImmunohistology: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2121-89P1

Size

100ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Tian, X et al; Identification of an angiogenic factor that when mutated causes susceptibility to Klippel-Trenaunay syndrome. Nature 2004, 427: 640.

Additional Info

Reacts with human VG5Q (Mr 84kD).