VGAT (Vesicular GABA Amino Acid Transporter, Vesicular GABA Transporter, bA122O1.1, GABA and Glycine Transporter, hVIAAT, Solute Carrier Family 32 (GABA Vesicular Transporter) Member 1, Solute Carrier Family 32 Member 1, SLC32A1, Vesicular Inhibitory Amin

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Acid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Rat hippocampal lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2121-87H

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Chaudhry FA, Reimer RJ, Bellocchio EE, Danbolt NC, Osen KK, Edwards RH, Storm-Mathisen J (1998) The vesicular GABA transporter, VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons. J Neurosci 18:9733-9750.De GS, Schafer MK, Defamie N, Chen C, Ricci A, Weihe E, Varoqui H, Erickson JD (2005) Homeostatic scaling of vesicular glutamate and GABA transporter expression in rat neocortical circuits. J Neurosci 25:7121-7133.McIntire SL, Reimer RJ, Schuske K, Edwards RH, Jorgensen EM (1997) Identification and characterization of the vesicular GABA transporter. Nature (Lond ) 389:870-876.

Additional Info

The antibody has been directly tested for reactivity in rat tissue. It is anticipated that the antibody will also work with bovine, canine and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.