VGAT (Vesicular GABA Amino Acid Transporter, Vesicular GABA Transporter, bA122O1.1, GABA and Glycine Transporter, hVIAAT, Solute Carrier Family 32 (GABA Vesicular Transporter) Member 1, Solute Carrier Family 32 Member 1, SLC32A1, Vesicular Inhibitory Amin

Synaptic transmission involves the regulated exocytosis of vesicles containing neurotransmitters. The inhibitory neurotransmitter g-aminobutyric acid (GABA) is synthesized in the cytoplasm from L-glutamate by the enzyme glutamic acid decarboxylase (GAD) and loaded in synaptic vesicles for exocytosis. The vesicular GABA transporter is responsible for the uptake of GABA into synaptic vesicles, following synthesis by GAD, or reuptake by plasma membrane GABA transporters (GATs). VGAT protein (55kD) contains ten transmembrane domains and belongs to a new family of vesicular neurotransmitter transporters. VGAT is highly conserved in mouse, rat and human (95-98% identity). VGAT uses both chemical and electrical components of the luminal protein gradient to mediate the accumulation of GABA into synaptic vesicles. VGAT is also responsible for glycine uptake in glycinergic neurons. VGAT is a homolog of the c. elegans unc -47 protein involved in GABA transport. Unc-47 is expressed in GABA neurons, localizes to synaptic vesicles and confers vesicular GABA transport in transfected cells. Mutants of c. elegans in which the unc-47 gene encoding VGAT is non-functional or absent, exhibit a complete loss of GABAergic function and elevated levels of GABA in the cytoplasm. VGAT is present in nerve endings of inhibitory neurons containing GABA, but also in glycinergic neurons in the brain and retina. At subcellular levels, VGAT specifically associates with synaptic vesicles. VGAT/VIAAT is also expressed in rat pancreatic islet cells.\n\nApplications: \nSuitable for use in Western Blot and Microarray. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2-4ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2121-87J

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. McIntire, S.L., et al., Identification and characterization of the vesicular GABA transporter., Nature, 389, 870-876 (1997). 2. Sagne, C., Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases., FEBS Lett., 417, 177-183 (1997). 3. Ebihara, S., et al., Mouse vesicular GABA transporter gene: genomic organization, transcriptional regulation and chromosomal localization., Brain Res. Mol. Brain Res., 110, 126- 139 (2003). 4. Jellali, A., et al., Cellular localization of the vesicular inhibitory amino acid transporter in the mouse and human retina. J. Comp. Neurol., 449, 76-87 (2002). 5. Chaudhry, F.A., et al., The vesicular GABA transporter, VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons., J. Neurosci., 18, 9733-9750 (1998). 6. Takamori, S., et al., Immunoisolation of GABAspecific synaptic vesicles defines a functionally distinct subset of synaptic vesicles., J. Neurosci., 20, 4904-4911 (2000). 7. Chessler, S.D., Expression of the vesicular inhibitory amino acid transporter in pancreatic islet cells: distribution of the transporter within rat islets. Diabetes, 51, 1763-1771 (2002)

Additional Info

Recognizes mouse VGAT at ~57kD. Species Crossreactivity: rat