Vesicular glutamate transporter (VGLUT) is responsible for the active transport of L-glutamate in synaptic vesicles and thus plays an essential role in the glutamatergic chemical transmission in the central nervous system. VGLUT comprises three isoforms, VGLUT1, 2, and 3, and is a potential marker for the glutamatergic phenotype. Recent studies indicated that VGLUT is also expressed in non-neuronal cells, and localized with various organelles such as synaptic-like microvesicles in the pineal gland, and hormone- containing secretory granules in endocrine cells. L-Glutamate is stored in these organelles, secreted upon various forms of stimulation, and then acts as a paracrine-like modulator. Thus, VGLUTs highlight a novel framework of glutamatergic signaling and reveal its diverse modes of action. VGLUT3 expression is restricted to the brain, where it is predominantly located in synaptic vesicles. VGLUT3 shows strong sequence homology (~73%) to Differentiation-associated Na- dependent inorganic phosphate cotransporter. Mouse and rat respectively show 90 and 92% identity with the human VGLUT3 protein sequence.
Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions:
Western Blot: 1-10ug/ml (Chemiluminescence)
ELISA: 1:100,000; 50-100ng of G4007-02A control peptide/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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