Villin 1

Villin is a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli. Phosphorylation of villin at Tyr60, 81 and 256 may be involved in the regulation of cell migration. Expression of villin is increased in colorectal cancers, and villin 1 function appears to be involved in progressive cholestasis and hepatic failure.\n\nApplications: \nSuitable for use in Immunofluorescence, Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence (IC): 1:100\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2121-95

Size

100ul

Applications

ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

Isotype

IgG

References

1. Athman, R., et al., Am. J. Physiol. Gastrointest. Liver Physiol. 283: G496-502 (2002). 2. Kumar, N., et al., J. Biol. Chem. 279: 45,036-45,046 (2004). 3. Tomar, A., et al., Mol. Biol. Cell 15: 4807-4817 (2004). 4. Suh, N., et al., Mod. Pathol. 18: 1217-1222 (2005). 5. Phillips, M.J., et al., Lancet 362: 1112-1119 (2003).

Additional Info

Recognizes endogenous levels of total human villin 1 at ~95kD. Species Crossreactivity: mouse