Villin

Villin can cap, nucleate, sever and bundle actin in a Ca2+ and phosphoinositideregulated manner. It is associated with the microvillar actin core bundle of intestinal and renal brush border implicated in adsorption. Villin is composed of six repeats, each containing 150 residues that together constitute the core domain followed by the carboxylterminal headpiece domain of 87 residues. The core domain retains the Ca2+ dependent capping nucleating and severing activity, whereas the headpiece domain contributes towards actin filament bundling and binding F-actin, independently of Ca2+. \n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution: \nImmunohistochemistry (formalin/paraffin): 1:50-1:100 for 30 min at RT\nNote: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Small intestine and kidney \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n

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