Villin can cap, nucleate, sever and bundle actin in a Ca2+ and phosphoinositide-regulated manner. It is associated with the microvillar actin core bundle of intestinal and renal brush border implicated in adsorption. Villin is composed of six repeats, each containing 150 residues that together constitute the core domain followed by the carboxyl-terminal headpiece domain of 87 residues. The core domain retains the Ca2+ dependent capping nucleating and severing activity, whereas the headpiece domain contributes towards actin filament bundling and binding F-actin, independently of Ca2+.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Positive Control:
Small intestine and kidney
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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