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Vimentin (FLJ36605, VIM)

Vimentin (FLJ36605, VIM)

Cat no: V2122-01B

Supplier: United States Biological
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Vimentin is protein subunit of the 10 nm or intermediate filaments protein found in many kinds of mesenchymal and epithelia cells, in many kinds of cells in tissue culture and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in many kinds of astrocytes), with desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Applications: Suitable for use in Immunofluorescence. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:1000 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1903-80: IgY, H&L, X-Adsorbed, 12nm (Gold) Pab Dk x Ch I1903-80A: IgY, H&L, X-Adsorbed, 18nm (Gold) Pab Dk x Ch I1903-80B: IgY, H&L, X-Adsorbed, 6nm (Gold) Pab Dk x Ch I1906: IgY Pab Rb x Ch I1906-01: IgY, Fc (HRP) Pab Gt x Ch I1906-10: IgY (HRP) Pab Rb x Ch I1906-12: IgY (FITC) Pab Rb x Ch I1906-14: IgY (AP) Pab Rb x Ch Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: V2122-01B
Reactivities: Human, Mouse, Rat
Hosts: Chicken/Bird
Applications: Immunofluorescence
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type: Pab
Isotype: IgY
Purity: Purified by immunoaffinity chromatography.
References: 1. Ermakova S, et al. The intermediate filament protein vimentin is a new target for epigallocatechin gallate. J. Biol. Chem. Apr 2005; 280(17): 16882-16890. 2. Ishizaki M, et al. Expression of collagen I, smooth muscle alpha-actin, and vimentin during the healing of alkali-burned and lacerated corneas. Invest. Ophthalmol. Vis. Sci. Nov 1993; 34(12): 3320-3328.
Additional info: Recognizes Vimentin. Species Crossreactivity: human, rat, mouse.

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