Vimentin (FLJ36605, VIM)

Supplier:

United States Biological

Cat no: V2122-09

Vimentin (FLJ36605, VIM)

Vimentin (57kD) is the intermediate filament protein (IFP) of mesenchymal cells. It is the only IFP, which deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.

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SPECIFICATIONS

Catalog Number

V2122-09

Size

100ug

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Ch/Bird, Goat, NHP

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

USBio product reference: 1. Cheng et al. Stem Cell Research & Therapy (2010) 1:6-13. 1. Ramaekers F, Huijsmans A, Schaart G, Moesker O, Vooijs P - Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res 170: 235-249 (1987). 2. Pieper FR, Schaart G, Krimpenfort PJ, Henderik JB, Moshage HJ, Van de Kemp A, Ramaekers FCS, Berns A, Bloemendal H - Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells. J Cell Biol 108: 1009-1024 (1989). 3. Viebahn C, Lane EB, Ramaekers FCS - Keratin and vimentin expression in early organogenesis of the rabbit embryo. Cell Tissue Res 253: 553-562 (1988). 4. Raats JMH, Pieper FR, Vree Egberts WTM, Verrijp KN, Ramaekers FCS, Bloemendal H - Assembly of amino-terminal deleted desmin and vimentin-free cells. J Cell Biol 111: 1971-1985 (1990). 5. Ramaekers FCS, Van Niekerk CC, Poels LG, Schaafsma E, Huijsmans A, Robben H, Schaart G, Vooijs GP - Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136 : 641-655 (1990).

Additional Info

Recognizes 57kD protein corresponding to vimentin in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. Species Crossreactivity: human, mouse, rat, hamster, chicken, goat, canine and monkey.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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