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Vimentin, phosphorylated (Ser72)

Vimentin, phosphorylated (Ser72)

Cat no: V2122-10J

Supplier: United States Biological
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Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed in during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5). Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1,000-1:10,000 Immunoprecipitation: 1:40 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: V2122-10J
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50 mM Tris- Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1.Eriksson JE et al. Curr. Opin. Cell Biol. 4:99-104, 1992.2.Inagaki M et al. Nature 328: 649-652, 1987.3.Goto H et al. GENES CELLS 7:91-97. 2002.4.Goto H et al. J Biol Chem, 273(19):11728-11736, 1998.5.Yamaguchi T et al. JCB, 171(3):431-436, 2005.
Additional info: Recognizes human Vimentin, phosphorylated (pS72). Detects Vimentin phosphorylated at Serine 72. Species Crossreactivity: mouse and rat.

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