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Vimentin (VIM, FLJ36605, OTTHUMP00000019224)

Vimentin (VIM, FLJ36605, OTTHUMP00000019224)

Cat no: V2122-01T

Supplier: United States Biological
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Vimentins are class-III intermediate filaments specific to mesenchymal tissue. Vimentin is an important cytoskeletal component responsible for maintaining cell integrity and has a probable role in the intracellular transport of proteins such as lipoproteins between the nucleus and plasma membrane. Immunohistochemical staining for Vimentin is characteristic of sarcomas. Applications: Suitable for use in Western Blot and Immunohisochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry (Paraffin and Formalin): 1.5-2.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: V2122-01T
Reactivities: Human, Mouse, Rat, Porcine
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with 1ml of PBS.
P type: Mab
Isotype: IgG
Purity: Purified by affinity chromatography.
Additional info: Recognizes porcine Vimentin at 54kD. Species Crossreactivity: human, mouse, rat.

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