Vinculin (BSA & Azide Free)

Vinculin is an attachment protein involved in the indirect binding of intracellular actin filaments to extracellular fibronectin. Vinculin binds to actin capping protein and alpha actinin, which in turn binds to actin. The binding of fibronectin is also indirect where vinculin binds to talin, which in turn binds to the intracellular domain of the fibronectin receptor which itself is the ligand for fibronectin. Vinculin is widely distributed in tissue and is expressed where smooth muscle actin and fibroblasts attach to the extracellular matrix. \n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Studies of vinculin in platelet activation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-2ug/ml for 2 hours at RT.\nImmunoprecipitation (Denatured): 2ug/mg protein lysate. Use Protein G.\nImmunohistochemistry (Formalin/paraffin): 1:50 for 20 minutes at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa cells, skin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2122-63X

Size

100ul

Applications

IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives or stabilizing proteins added. Also available with BSA and azide. See V2122-63.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Asijee, G.M., et al., European Journal of Biochemistry. 189: 131-136, 1990. 2. Molecular Biology of the Cell. 2nd Edition. Garland Publishing Inc. Page 636.

Additional Info

Recognizes human vinculin.