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Vinculin (Metavinculin, VCL)

Cat no: V2122-63H


Supplier: United States Biological
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Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066aa and, furthermore, the 2 proteins exhibit a high level of sequence identity (greater than 95%). Vinculinis mapped to 10q22.1-q23. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml detects vinculin in cells and tissues Immunohistochemistry (frozen): 2-4ug/ml on formalin or acetone-fixed tissues Optimal dilutions to be determined by the researcher . Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: V2122-63H
Reactivities: Human, Mouse, Rat, Chicken/Bird
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.
P type: Mab
Isotype: IgG1
Purity: Purified by immunoaffinity chromatography.
References: 1. Weller P A, et al., Proc. Nat Acad SCi. 87: 5667-5671 (1990) 2. Mulligan, L.M. et al., Genomics 13:1347-1349 (1992)
Additional info: Recognizes human Vinculin. Species Crossreactivity: mouse, rat, chicken.

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