Vinculin/VCL (Metavinculin)

In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is \ninvolved in linkage of integrin adhesion molecules to the actin cytoskeleton. By linkage studies in a \n3-generation family, Mulligan et al. (1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to \nD10S22.Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is \nthought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. \nMultiple alternatively-spliced transcript variants have been found for this gene, but the biological validity \nof some variants has not been determined. Human vinculin protein exhibits a greater-than-95% \nsequence identity to the chicken vinculin protein.Vinculin is a 117-kDa cytoskeletal protein with 1066 \namino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain \nseparated by a proline-rich middle segment. Vinculin consists of a globular head domain that contains \nbinding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region \ncontains binding sites for F-actin, paxillin, and lipids.\n\nUniProt Number:\nP18206\n\nGene ID:\nVCL\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (Paraffin).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145357

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bakolitsa, C., Cohen, D. M., Bankston, L. A., Bobkov, A. A., Cadwell, G. W., Jennings, L., Critchley, \nD. R., Craig, S. W., Liddington, R. C.Structural basis for vinculin activation at sites of cell \nadhesion.Nature 430: 583-586, 2004.\n2. Mulligan, L. M., Gardner, E., Telenius, H., Ponder, B. A. J.Complementary physical and genetic \ntechniques map the vinculin (VCL) gene on chromosome 10q.Genomics 13: 1347-1349, 1992.\n3. Vasile, V. C., Edwards, W. D., Ommen, S. R., Ackerman, M. J.Obstructive hypertrophic \ncardiomyopathy is associated with reduced expression of vinculin in the intercalated disc.Biochem. \nBiophys. Res. Commun. 349: 709-715, 2006.

Additional Info

Recognizes mouse Vinculin/VCL. No crossreactivity with other proteins.