Vinculin (VCL, VINC, CMD1W, CMH15, Metavinculin, MVCL)

Cat no: V2122-63N

Supplier: United States Biological

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Description
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Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix adherens type junctions. Multiple isoforms are produced by alternative splicing. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Immunohistochemistry: 2-4ug/ml in formalin/acetone fixed frozen tissues. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	V2122-63N
Reactivities:	Human, Mouse, Rat, Chicken/Bird
Hosts:	Mouse
Applications:	Immunohistochemistry, Western Blot
Size:	100ug
Form:	Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodum azide. Reconstitute with 1ml of PBS, pH 7.4.
P type:	Mab
Isotype:	IgG
Purity:	Purified by Protein G affinity chromatography.
Alternative names:	VIN 1
Additional info:	Recognizes human Vinculin at ~124kD. Species Crossreactivity: mouse, rat and chicken

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Vinculin (VCL, VINC, CMD1W, CMH15, Metavinculin, MVCL)

Cat no: V2122-63N

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